This is an unpublished mouse strain in which the first exon of Pdx1 was replaced with coding sequences for Cre recombinase by gene targeting.  The mouse was developed by Mark Magnuson. 

Keywords: Cre Pdx1 Mgn

Record History
Added on July 7, 2015 at 10:28 AM by Magnuson, Mark
Modified on December 6, 2018 at 9:34 AM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository

Mouse Information

Common Name Pdx1Cre
Research Applications Not provided
MMRRC ID Not provided
Jackson Laboratories Stock No Not provided
VCMR ID OV
Additional Strain Information

An 8.62 kb region of this gene has been replaced by tandemly oriented Lox66 and Lox2272 sites flanking positive (puromycin) and negative (HSV-TK) selectable markers.

The Pdx1.Cre exchange vector was made by cloning Cre coding sequences immediately following the translation initiation site in the Pdx1 gene.  The basal Pdx1 exchange vector contains all of the 8,610 bp of DNA from Pdx1 gene (promoter and exon 1 that is absent in the Pdx1LCA allele, Lox66 and Lox2272 sites, and a flrted (flanked by FRT) hygromycin selection cassette.

Genetic Alteration

Mutation #1: RMCE Targeted Mutagenesis
Type of AlleleCassette Acceptor
Targeted GeneName: pancreatic and duodenal homeobox 1
Symbol: Pdx1
NCBI: 18609
Allele Name: pancreatic and duodenal homeobox 1; cre
Symbol: Pdx1cre
Description of Targeting Vector

An 8.62 kb region of this gene has been replaced by tandemly oriented Lox66 and Lox2272 sites flanking positive (puromycin) and negative (HSV-TK) selectable markers.


Vector Genbank File Not provided
Allele Map Not Provided
PCR Genotyping Protocol Pdx1.Cre_PCR_genotyping_protocol.docx
Type of Allele Gene Replacement
Exchanged Cassette Gene Name pancreatic and duodenal homeobox 1 (Pdx1)
Exchanged Cassette Allele Name Pdx1{Cre}
Description of Exchange Vector

The Pdx1.Cre exchange vector was made by cloning Cre coding sequences immediately following the translation initiation site in the Pdx1 gene.  The basal Pdx1 exchange vector contains all of the 8,610 bp of DNA from Pdx1 gene (promoter and exon 1 that is absent in the Pdx1LCA allele, Lox66 and Lox2272 sites, and a flrted (flanked by FRT) hygromycin selection cassette.

Exchanged Cassette Genbank File Pdx1.Cre.gb
PCR Genotyping Protocol Not provided
Citations Not provided

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Current Genetic Background C57BL/6J
Number of Generations Backcrossed Not provided
Strain Description

93.75% C57Bl6/J at cryopreservation

Cryopreserved in 2013.

 

Attachment

Document pdx1crehygro.png - Added on July 7, 2015 at 10:28 AM by Mark Magnuson

The Pdx1.cre vector was exchanged into Pdx1LCA mESCs utilizing Recombinase Mediated Cassette Exchange (RMCE) and mice were generated from the correctly exchanged mESCs.  These animals were subsequently mated with Flpe mice in order to remove the HygroR cassette.