|Method of Cryopreservation||Sperm|
|Trial IVF % Fertilization||98.39%|
|Mutation #1: RMCE Targeted Mutagenesis|
Name: pancreatic and duodenal homeobox 1; targeted mutation 1.1, Christopher VE Wright
|Zygosity at cryopreservation||Heterozygote|
|PCR Genotyping Protocol||Pdx1tm1.1Cvw_PCR_Genotyping_Protocol_020818.docx|
|Chimera/Founder Genetic Background||129S6/SvEvTac|
|Cryopreservation Strain Background (VCMR)||C57BL/6J|
|Viability and Fertility Data||
Normal litters (8-9 pups per litter).
This line is viable other than the mice become diabetic when they are approximately 6-8 months old.
An 8.62 kb region of this gene has been replaced by tandemly oriented Lox66 and Lox2272 sites flanking positive (puromycin) and negative (HSV-TK) selectable markers.
The pdx1 Area II enhancer region was deleted from the pPdx1.Ex1 basal exchange vector (255 bp from -2168/-1913 relative to the primary transcription start site reported by Gerrish, K.et.al., JBC 2000, PMID:10652343). This will replace endogenous pdx1 sequences, and allow for assessment of the role of this enhancer region in pdx1 expression in vivo. Sequences flanking Area II deletion: ctcatcctgt/gaaggaaggt