Common Name | Pdx1deltaII |
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VCMR ID | XY |
Date Cryopreserved | 2018-02-06 |
Method of Cryopreservation | Sperm |
Trial IVF % Fertilization | 98.39% |
Mutation #1: RMCE Targeted Mutagenesis | ||||
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Allele |
Name: pancreatic and duodenal homeobox 1; targeted mutation 1.1, Christopher VE Wright Symbol: Pdx1tm1.1Cvw MGI: MGI:5823773 |
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Zygosity at cryopreservation | Heterozygote | |||
PCR Genotyping Protocol | Pdx1tm1.1Cvw_PCR_Genotyping_Protocol_020818.docx | |||
Citations |
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Strain Type | Mixed |
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Chimera/Founder Genetic Background | 129S6/SvEvTac |
Cryopreservation Strain Background (VCMR) | C57BL/6J |
Viability and Fertility Data | Normal litters (8-9 pups per litter). This line is viable other than the mice become diabetic when they are approximately 6-8 months old.
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An 8.62 kb region of this gene has been replaced by tandemly oriented Lox66 and Lox2272 sites flanking positive (puromycin) and negative (HSV-TK) selectable markers.
Pdx1AIId.png - Added on July 7, 2015 at 10:27 AM by Mark Magnuson
The pdx1 Area II enhancer region was deleted from the pPdx1.Ex1 basal exchange vector (255 bp from -2168/-1913 relative to the primary transcription start site reported by Gerrish, K.et.al., JBC 2000, PMID:10652343). This will replace endogenous pdx1 sequences, and allow for assessment of the role of this enhancer region in pdx1 expression in vivo. Sequences flanking Area II deletion: ctcatcctgt/gaaggaaggt