These mice, which contain a targeted deletion of Area II of Pdx1, provide a model for the central role of AreaII in the Pdx1 transcription in pancreatic organogenesis and beta cell differentiation.

 


Record History
Added on July 7, 2015 at 10:27 AM by Magnuson, Mark
Modified on December 7, 2018 at 10:33 AM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository

Mouse Information

Common Name Pdx1deltaII
Research Applications Not provided
MMRRC ID Not provided
Jackson Laboratories Stock No Not provided
VCMR ID XY
Additional Strain Information

An 8.62 kb region of this gene has been replaced by tandemly oriented Lox66 and Lox2272 sites flanking positive (puromycin) and negative (HSV-TK) selectable markers.

Genetic Alteration

Mutation #1: RMCE Targeted Mutagenesis
Type of AlleleRMCE Pdx1 AreaII Deletion
Targeted GeneName: pancreatic and duodenal homeobox 1
Symbol: Pdx1
NCBI: 18609
Allele Name: pancreatic and duodenal homeobox 1; targeted mutation 1.1, Christopher VE Wright
Symbol: Pdx1tm1.1Cvw
MGI: MGI:5823773
Description of Targeting Vector

An 8.62 kb region of this gene has been replaced by tandemly oriented Lox66 and Lox2272 sites flanking positive (puromycin) and negative (HSV-TK) selectable markers.


Vector Genbank File Not provided
Allele Map Not Provided
PCR Genotyping Protocol Pdx1tm1.1Cvw_PCR_Genotyping_Protocol_020818.docx
Type of Allele Gene Replacement
Exchanged Cassette Gene Name ()
Exchanged Cassette Allele Name Pdx1{AIId}
Description of Exchange Vector

The pdx1 Area II enhancer region was deleted from the pPdx1.Ex1 basal exchange vector (255 bp from -2168/-1913 relative to the primary transcription start site reported by Gerrish, K.et.al., JBC 2000). This will replace endogenous pdx1 sequences, and allow for assessment of the role of this enhancer region in pdx1 expression in vivo. Sequences flanking Area II deletion: ctcatcctgt/gaaggaaggt

Exchanged Cassette Genbank File pdx1AIId.gb
PCR Genotyping Protocol Not provided
Citations
Publication
The mammal-specific Pdx1 Area II enhancer has multiple essential functions in early endocrine cell specification and postnatal β-cell maturation. (2017) Development 144: 248-257 (Added 12/29/2016)
PMID: 27993987
Pancreatic beta cell-specific transcription of the pdx-1 gene. The role of conserved upstream control regions and their hepatic nuclear factor 3beta sites. (2000) J Biol Chem 275: 3485-92 (Added 1/6/2014)
PMID: 10652343

Background Strain Information

Strain Type Mixed
Chimera/Founder Genetic Background 129S6/SvEvTac
Current Genetic Background C57BL/6J
Number of Generations Backcrossed Not provided
Strain Description Not provided

Attachment

Document Pdx1AIId.png - Added on July 7, 2015 at 10:27 AM by Mark Magnuson

The pdx1 Area II enhancer region was deleted from the pPdx1.Ex1 basal exchange vector (255 bp from -2168/-1913 relative to the primary transcription start site reported by Gerrish, K.et.al., JBC 2000, PMID:10652343). This will replace endogenous pdx1 sequences, and allow for assessment of the role of this enhancer region in pdx1 expression in vivo.  Sequences flanking Area II deletion: ctcatcctgt/gaaggaaggt