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B6;129S6-Sox17tm1.2(cre/ERT2)Mgn/Vu

This is an unpublished line that expresses CreERT2 under control of the Sox17 gene locus.  These mice can be used for lineage tracing of Sox17 gene expression by tamoxifen injection.  The line is highly similar in design to Sox17-CreGFP mice which has been published.  The line was generated by Mark Magnuson in collaboration with Anne Grapin-Botton.

Record History
Added on July 7, 2015 at 10:27 AM by Magnuson, Mark
Modified on September 5, 2019 at 12:20 PM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository
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Mouse Information

Common Name Sox17CreERT2
VCMR ID LC
Date Cryopreserved 2010-04-20
Method of Cryopreservation Sperm
Trial IVF % Fertilization 7.89%

Genetic Alteration

Mutation #1: RMCE Targeted Mutagenesis
Allele Name: targeted mutation 1.2
Symbol: Sox17tm1.2(cre/ERT2)Mgn
Zygosity at cryopreservation Heterozygote
PCR Genotyping Protocol Sox17CreER_genotyping_protocol.docx
Citations Not provided

Background Strain Information

Strain Type Mixed
Chimera/Founder Genetic Background 129S6/SvEvTac
Cryopreservation Strain Background (VCMR) C57BL/6J
Viability and Fertility Data

Strain Background:  87.5% C57Bl6/J at cryopreservation.

Homozygous embryonic lethal at E10.5.

Heterozygous fertile and viable.

Additional Information

pSox17.LCA targeting vector contains 10.288 kb 5’ arm and 4.525 kb5 3’arm.  Lox66 and Lox2272 sites are inserted flanking PuTK selection marker for positive selection for targeting events with puromycin and negative selection for RMCE events with ganciclovir.

The exchange vector used for RMCE replaces Sox17 coding sequences with CreERT2. The vector also contains a Pgk-hygromycin resistance cassette flanked by tandem FRT sites, enabling excision of the cassette after germline transmission.

Attachment

Document Sox17.CreERT2.png - Added on July 7, 2015 at 10:27 AM by Mark Magnuson

Map of RMCE exchange vector used to derive this line.