This is an unpublished line that expresses CreERT2 under control of the Sox17 gene locus.  These mice can be used for lineage tracing of Sox17 gene expression by tamoxifen injection.  The line is highly similar in design to Sox17-CreGFP mice which has been published.  The line was generated by Mark Magnuson in collaboration with Anne Grapin-Botton.


Record History
Added on July 7, 2015 at 10:27 AM by Magnuson, Mark
Modified on September 5, 2019 at 12:20 PM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository

Mouse Information

Common Name Sox17CreERT2
Research Applications Not provided
MMRRC ID Not provided
Jackson Laboratories Stock No Not provided
VCMR ID LC
Additional Strain Information

pSox17.LCA targeting vector contains 10.288 kb 5’ arm and 4.525 kb5 3’arm.  Lox66 and Lox2272 sites are inserted flanking PuTK selection marker for positive selection for targeting events with puromycin and negative selection for RMCE events with ganciclovir.

The exchange vector used for RMCE replaces Sox17 coding sequences with CreERT2. The vector also contains a Pgk-hygromycin resistance cassette flanked by tandem FRT sites, enabling excision of the cassette after germline transmission.

Genetic Alteration

Mutation #1: RMCE Targeted Mutagenesis
Type of AlleleCassette Acceptor
Targeted GeneName: SRY-box containing gene 17
Symbol: Sox17
NCBI: 20671
Allele Name: targeted mutation 1.2
Symbol: Sox17tm1.2(cre/ERT2)Mgn
Description of Targeting Vector

pSox17.LCA e targeting vector contains 10.288 kb 5’ arm and 4.525 kb5 3’arm.  Lox66 and Lox2272 sites are inserted flanking PuTK selection marker for positive selection for targeting events with puromycin and negative selection for RMCE events with ganciclovir.


Vector Genbank File Not provided
Allele Map Not Provided
PCR Genotyping Protocol Sox17CreER_genotyping_protocol.docx
Type of Allele Global Null
Exchanged Cassette Gene Name (CreERT2)
Exchanged Cassette Allele Name Sox17{CreERT2}
Description of Exchange Vector

The exchange vector used for RMCE replaces Sox17 coding sequences with CreERT2. The vector also contains a Pgk-hygromycin resistance cassette flanked by tandem FRT sites, enabling excision of the cassette after germline transmission.

Exchanged Cassette Genbank File pBSLoxSox17CreERT2Hygro.gb
PCR Genotyping Protocol Not provided
Citations Not provided

Background Strain Information

Strain Type Mixed
Chimera/Founder Genetic Background 129S6/SvEvTac
Current Genetic Background C57BL/6J
Number of Generations Backcrossed 3
Strain Description

87.5% C57Bl6/J at cryopreservation.

Cryopreserved in 2010.

 

Attachment

Document Sox17.CreERT2.png - Added on July 7, 2015 at 10:27 AM by Mark Magnuson

Map of RMCE exchange vector used to derive this line.