Common Name | Sst.rTTA.LCA |
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VCMR ID | SD |
Date Cryopreserved | 2014-05-13 |
Method of Cryopreservation | Sperm |
Trial IVF % Fertilization | 74.63% |
Mutation #1: Targeted Mutagenesis | |
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Allele |
Name: Sst{rTTA.LCA.Mgn} Symbol: SstrTTA.LCA.Mgn |
Zygosity at cryopreservation | Heterozygote |
PCR Genotyping Protocol | Sst.rtTA_PCR_genotyoping_protocol.docx |
Citations | Not provided |
Strain Type | Congenic Strain |
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Chimera/Founder Genetic Background | 129S6/SvEvTac |
Cryopreservation Strain Background (VCMR) | C57BL/6J |
Viability and Fertility Data | Strain background: 96.8% C57Bl6/J at cryopreservation. Expected to be homozygous viable and fertile. Heterozygous viable and fertile. |
The targeting vector contains 7.3 Kb 5’ and a 3.6 kb 3’ homology arms. Lox66 and Lox2272 sites were inserted around a 500 bp region of the of Sst promoter and exons I and II of the somatostatin gene. rTTA coding sequences with a beta-globin polyA site replaced exons I and II of the Sst. The vector also contains FRT-flanked puTK-EM7 Neo selection double selection cassette. PU(delta)TK was used for positive selection for targeting events with puromycin and negative selection for RMCE events with ganciclovir. EM7-Neo was used for positive selection in bacteria during BAC recombineering process.
SstrTTA.LCA_GT_-Puro.png - Added on July 7, 2015 at 10:26 AM by Mark Magnuson
Gene targeting strategy.