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B6.129S6-SstrTTA.LCA.Mgn/Vu

This line expresses the reverse tetracycline-TransActivator (rtTA) under control of the somatostatin gene locus. The mice can be used to drive Tet-responsive gene expression in somatostatin expressing cells. In addition, during gene targeting Lox66 and Lox2272 sites were inserted, enabling the cells to be used for RMCE. These mice remain unpublished but are being used in at least two ongoing studies.

Record History
Added on July 7, 2015 at 10:26 AM by Magnuson, Mark
Modified on October 31, 2019 at 2:32 PM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository
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Mouse Information

Common Name Sst.rTTA.LCA
VCMR ID SD
Date Cryopreserved 2014-05-13
Method of Cryopreservation Sperm
Trial IVF % Fertilization 74.63%

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Allele Name: Sst{rTTA.LCA.Mgn}
Symbol: SstrTTA.LCA.Mgn
Zygosity at cryopreservation Heterozygote
PCR Genotyping Protocol Sst.rtTA_PCR_genotyoping_protocol.docx
Citations Not provided

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Cryopreservation Strain Background (VCMR) C57BL/6J
Viability and Fertility Data

Strain background:  96.8% C57Bl6/J at cryopreservation.

Expected to be homozygous viable and fertile.

Heterozygous viable and fertile.

Publications / Citations

  1. Pancreatic islet-autonomous insulin and smoothened-mediated signalling modulate identity changes of glucagon α-cells. Cigliola V, Ghila L, Thorel F, van Gurp L, Baronnier D, Oropeza D, Gupta S, Miyatsuka T, Kaneto H, Magnuson MA, Osipovich AB, Sander M, Wright CEV, Thomas MK, Furuyama K, Chera S, Herrera PL (2018) Nat Cell Biol 20(11): 1267-1277
    › Primary publication · 30361701 (PubMed) · PMC6215453 (PubMed Central) · Added on 11/6/2018

MeSH Terms

Animals Cell Differentiation Cell Plasticity Cell Proliferation Female Glucagon-Secreting Cells Insulin Insulin-Secreting Cells Islets of Langerhans Male Mice, Inbred C57BL Mice, Knockout Mice, SCID Mice, Transgenic Signal Transduction Smoothened Receptor

Additional Information

The targeting vector contains 7.3 Kb 5’ and a 3.6 kb 3’ homology arms.  Lox66 and Lox2272 sites were inserted around a 500 bp region of the of Sst promoter and exons I and II of the somatostatin gene. rTTA coding sequences with a beta-globin polyA site replaced exons I and II of the Sst.  The vector also contains FRT-flanked puTK-EM7 Neo selection double selection cassette. PU(delta)TK was used for positive selection for targeting events with puromycin and negative selection for RMCE events with ganciclovir.  EM7-Neo was used for positive selection in bacteria during BAC recombineering process.

Attachment

Document SstrTTA.LCA_GT_-Puro.png - Added on July 7, 2015 at 10:26 AM by Mark Magnuson

Gene targeting strategy.