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|Research Applications||Not provided|
|MMRRC ID||Not provided|
|Jackson Laboratories Stock No||Not provided|
|Additional Strain Information||
The targeting vector contains 7.3 Kb 5’ and a 3.6 kb 3’ homology arms. Lox66 and Lox2272 sites were inserted around a 500 bp region of the of Sst promoter and exons I and II of the somatostatin gene. rTTA coding sequences with a beta-globin polyA site replaced exons I and II of the Sst. The vector also contains FRT-flanked puTK-EM7 Neo selection double selection cassette. PU(delta)TK was used for positive selection for targeting events with puromycin and negative selection for RMCE events with ganciclovir. EM7-Neo was used for positive selection in bacteria during BAC recombineering process.
|Mutation #1: Targeted Mutagenesis|
|Type of Allele||Gene Replacement|
|Targeted Gene||Name: Somatostatin|
|Description of Targeting Vector||
The targeting vector contains 7.3 Kb 5’ and a 3.6 kb 3’ homology arms. Lox66 and Lox2272 sites are inserted flanking 500bp of Sst promoter region and exons I and II of somatostatin gene. rTTA gene with beta-globin polyA site is put in place of Sst exons I and II. The vector also contains FRT-flanked puTK-EM7 Neo selection double selection casstete. PuTK is used for positive selection for targeting events with puromycin and negative selection for RMCE events with ganciclovir. EM7-Neo is used for positive selection in bacteria duirng BAC recombineering process.
|Vector Genbank File||
|Allele Map||Not Provided|
|PCR Genotyping Protocol||Sst.rtTA_PCR_genotyoping_protocol.docx|
|Strain Type||Congenic Strain|
|Chimera/Founder Genetic Background||129S6/SvEvTac|
|Current Genetic Background||C57BL/6J|
|Number of Generations Backcrossed||3|
Germline male chimeras were mated to C57BL6/J female mice and positive offspring were identified. These offspring were subsequently backcrossed to C57BL6/J animals for a total of three generations.
96.8% C57Bl6/J at cryopreservation
Cryopreserved in 2014.
Trial IVF 74.63% fertilization rate.