This line expresses the reverse tetracycline-TransActivator (rtTA) under control of the somatostatin gene locus. The mice can be used to drive Tet-responsive gene expression in somatostatin expressing cells. In addition, during gene targeting Lox66 and Lox2272 sites were inserted, enabling the cells to be used for RMCE. These mice remain unpublished but are being used in at least two ongoing studies.


Record History
Added on July 7, 2015 at 10:26 AM by Magnuson, Mark
Modified on December 6, 2018 at 9:36 AM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository

Mouse Information

Common Name Sst.rTTA.LCA
Research Applications Not provided
MMRRC ID Not provided
Jackson Laboratories Stock No Not provided
VCMR ID SD
Additional Strain Information

The targeting vector contains 7.3 Kb 5’ and a 3.6 kb 3’ homology arms.  Lox66 and Lox2272 sites were inserted around a 500 bp region of the of Sst promoter and exons I and II of the somatostatin gene. rTTA coding sequences with a beta-globin polyA site replaced exons I and II of the Sst.  The vector also contains FRT-flanked puTK-EM7 Neo selection double selection cassette. PU(delta)TK was used for positive selection for targeting events with puromycin and negative selection for RMCE events with ganciclovir.  EM7-Neo was used for positive selection in bacteria during BAC recombineering process.

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Type of AlleleGene Replacement
Targeted GeneName: Somatostatin
Symbol: Sst
NCBI: 20604
Allele Name: Sst{rTTA.LCA.Mgn}
Symbol: SstrTTA.LCA.Mgn
Description of Targeting Vector

The targeting vector contains 7.3 Kb 5’ and a 3.6 kb 3’ homology arms.  Lox66 and Lox2272 sites are inserted flanking 500bp of Sst promoter region and exons I and II of somatostatin gene. rTTA gene with beta-globin polyA site is put in place of Sst exons I and II.  The vector also contains FRT-flanked puTK-EM7 Neo selection double selection casstete. PuTK is used for positive selection for targeting events with puromycin and negative selection for RMCE events with ganciclovir. EM7-Neo is used for positive selection in bacteria duirng BAC recombineering process.


Vector Genbank File pSst.rtTA.LCA.gb
Allele Map Not Provided
PCR Genotyping Protocol Sst.rtTA_PCR_genotyoping_protocol.docx
Citations Not provided

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Current Genetic Background C57BL/6J
Number of Generations Backcrossed 3
Strain Description

Germline male chimeras were mated to C57BL6/J female mice and positive offspring were identified.  These offspring were subsequently backcrossed to C57BL6/J animals for a total of three generations.

96.8% C57Bl6/J at cryopreservation

Cryopreserved in 2014.

Trial IVF 74.63% fertilization rate.

Publications / Citations

  1. Pancreatic islet-autonomous insulin and smoothened-mediated signalling modulate identity changes of glucagon α-cells. Cigliola V, Ghila L, Thorel F, van Gurp L, Baronnier D, Oropeza D, Gupta S, Miyatsuka T, Kaneto H, Magnuson MA, Osipovich AB, Sander M, Wright CEV, Thomas MK, Furuyama K, Chera S, Herrera PL (2018) Nat Cell Biol 20(11): 1267-1277
    › Primary publication · 30361701 (PubMed) · PMC6215453 (PubMed Central) · Added on 11/6/2018

MeSH Terms

Animals Cell Differentiation Cell Plasticity Cell Proliferation Female Glucagon-Secreting Cells Insulin Insulin-Secreting Cells Islets of Langerhans Male Mice, Inbred C57BL Mice, Knockout Mice, SCID Mice, Transgenic Signal Transduction Smoothened Receptor

Attachment

Document SstrTTA.LCA_GT_-Puro.png - Added on July 7, 2015 at 10:26 AM by Mark Magnuson

Gene targeting strategy.