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B6;129S6-Ptf1atdTomatoMgn/Vu

This is an unpublished mouse strain.  This mouse cell line was generated by RMCE in the Ptf1a[LCA] allele. Pancreata of this mouse line express both td.Tomato (a red fluorescent protein) and an N-terminal epitope tagged Strep/Flag (NSF) fusion of the transcription factor Ptf1a. This line may be used in a variety of applications such as FACS sorting of Ptf1a expressing cells, chromatin immunoprecipitation, and proteomic analyses.

Record History
Added on July 7, 2015 at 10:25 AM by Magnuson, Mark
Modified on September 3, 2019 at 8:13 AM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository
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Mouse Information

Common Name Ptf1atdTomato
VCMR ID MB
Date Cryopreserved 2011-05-03
Method of Cryopreservation Sperm
Trial IVF % Fertilization 39.20%

Genetic Alteration

Mutation #1: RMCE Targeted Mutagenesis
Allele Name: Ptf1a{tdTomato}
Symbol: Ptf1atdTomato
Zygosity at cryopreservation Heterozygote
PCR Genotyping Protocol Ptf1a.tdTomato_PCR_genotyping_protocol.docx
Citations Not provided

Background Strain Information

Strain Type Mixed
Chimera/Founder Genetic Background 129S6/SvEvTac
Cryopreservation Strain Background (VCMR) C57BL/6J
Viability and Fertility Data

Strain background:  87.5% C57Bl6/J at cryopreservation

Homozygous viable and fertile.

Heterozygous viable and fertile.

Additional Information

The Ptf1a.tdTOM-NSF-p48.KO.hygro vector was made on a backbone of a basal exchange vector which contains a 4.105 kb sequence from the Ptf1a locus, inverted loxP sites, and a flrted (flanked by FRT) Pgk-Hygro cassette that is used for positive selection of ES cells after RMCE.  Sequences for td.Tomato (a red fluorescent protein) and an N-terminal epitope tagged Strep/Flag (NSF) fusion of the transcription factor Ptf1a were cloned dowstream of the Ptf1a ATG site.

Attachment

Document Ptf1a.tdTomato.NSF-hygro.png - Added on July 7, 2015 at 10:25 AM by Mark Magnuson