Common Name | Ptf1atdTomato |
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VCMR ID | MB |
Date Cryopreserved | 2011-05-03 |
Method of Cryopreservation | Sperm |
Trial IVF % Fertilization | 39.20% |
Mutation #1: RMCE Targeted Mutagenesis | |
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Allele |
Name: Ptf1a{tdTomato} Symbol: Ptf1atdTomato |
Zygosity at cryopreservation | Heterozygote |
PCR Genotyping Protocol | Ptf1a.tdTomato_PCR_genotyping_protocol.docx |
Citations | Not provided |
Strain Type | Mixed |
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Chimera/Founder Genetic Background | 129S6/SvEvTac |
Cryopreservation Strain Background (VCMR) | C57BL/6J |
Viability and Fertility Data | Strain background: 87.5% C57Bl6/J at cryopreservation Homozygous viable and fertile. Heterozygous viable and fertile. |
The Ptf1a.tdTOM-NSF-p48.KO.hygro vector was made on a backbone of a basal exchange vector which contains a 4.105 kb sequence from the Ptf1a locus, inverted loxP sites, and a flrted (flanked by FRT) Pgk-Hygro cassette that is used for positive selection of ES cells after RMCE. Sequences for td.Tomato (a red fluorescent protein) and an N-terminal epitope tagged Strep/Flag (NSF) fusion of the transcription factor Ptf1a were cloned dowstream of the Ptf1a ATG site.