|Method of Cryopreservation||Sperm|
|Trial IVF % Fertilization||39.20%|
|Mutation #1: RMCE Targeted Mutagenesis|
|Zygosity at cryopreservation||Heterozygote|
|PCR Genotyping Protocol||Ptf1a.tdTomato_PCR_genotyping_protocol.docx|
|Chimera/Founder Genetic Background||129S6/SvEvTac|
|Cryopreservation Strain Background (VCMR)||C57BL/6J|
|Viability and Fertility Data||
Strain background: 87.5% C57Bl6/J at cryopreservation
Homozygous viable and fertile.
Heterozygous viable and fertile.
The Ptf1a.tdTOM-NSF-p48.KO.hygro vector was made on a backbone of a basal exchange vector which contains a 4.105 kb sequence from the Ptf1a locus, inverted loxP sites, and a flrted (flanked by FRT) Pgk-Hygro cassette that is used for positive selection of ES cells after RMCE. Sequences for td.Tomato (a red fluorescent protein) and an N-terminal epitope tagged Strep/Flag (NSF) fusion of the transcription factor Ptf1a were cloned dowstream of the Ptf1a ATG site.