This is an unpublished mouse strain.  This mouse cell line was generated by RMCE in the Ptf1a[LCA] allele. Pancreata of this mouse line express both td.Tomato (a red fluorescent protein) and an N-terminal epitope tagged Strep/Flag (NSF) fusion of the transcription factor Ptf1a. This line may be used in a variety of applications such as FACS sorting of Ptf1a expressing cells, chromatin immunoprecipitation, and proteomic analyses.


Record History
Added on July 7, 2015 at 10:25 AM by Magnuson, Mark
Modified on January 28, 2019 at 4:44 PM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository

Mouse Information

Common Name Ptf1atdTomato
Research Applications Not provided
MMRRC ID Not provided
Jackson Laboratories Stock No Not provided
VCMR ID MB
Additional Strain Information

The Ptf1a.tdTOM-NSF-p48.KO.hygro vector was made on a backbone of a basal exchange vector which contains a 4.105 kb sequence from the Ptf1a locus, inverted loxP sites, and a flrted (flanked by FRT) Pgk-Hygro cassette that is used for positive selection of ES cells after RMCE.  Sequences for td.Tomato (a red fluorescent protein) and an N-terminal epitope tagged Strep/Flag (NSF) fusion of the transcription factor Ptf1a were cloned dowstream of the Ptf1a ATG site.

Genetic Alteration

Mutation #1: RMCE Targeted Mutagenesis
Type of AlleleCassette Acceptor
Targeted GeneName: pancreas specific transcription factor, 1a
Symbol: Ptf1a
NCBI: 19213
Allele Name: Ptf1a{tdTomato}
Symbol: Ptf1atdTomato
Description of Targeting Vector Not provided
Vector Genbank File Not provided
Allele Map Not Provided
PCR Genotyping Protocol Ptf1a.tdTomato_PCR_genotyping_protocol.docx
Type of Allele Gene Replacement
Exchanged Cassette Gene Name ()
Exchanged Cassette Allele Name Ptf1a{td.TOM}
Description of Exchange Vector

The Ptf1a.tdTOM-NSF-p48.KO.hygro vector was made on a backbone of a basal exchange vector which contains a 4.105 kb sequence from the Ptf1a locus, inverted loxP sites, and a flrted (flanked by FRT) Pgk-Hygro cassette that is used for positive selection of ES cells after RMCE.  Sequences for td.Tomato (a red fluorescent protein) and an N-terminal epitope tagged Strep/Flag (NSF) fusion of the transcription factor Ptf1a were cloned dowstream of the Ptf1a ATG site.

Exchanged Cassette Genbank File p48.tdtomatonsfp48.ko.hygro.gb
PCR Genotyping Protocol Not provided
Citations Not provided

Background Strain Information

Strain Type Mixed
Chimera/Founder Genetic Background 129S6/SvEvTac
Current Genetic Background C57BL/6
Number of Generations Backcrossed Not provided
Strain Description

87.5% C57Bl6/J at cryopreservation

Cryopreserved in 2011.

Attachment

Document Ptf1a.tdTomato.NSF-hygro.png - Added on July 7, 2015 at 10:25 AM by Mark Magnuson