|Start Date / Time||July 22, 2015 at 9:00 AM|
|End Date / Time||July 22, 2015 at 9:55 AM|
|Location||512 Light Hall|
|Presenter Name||Hannah Worchel (Magnuson's lab)|
|Presentation Title||Inflammation Diverts Transcription Factor-mediated Acinar to Beta Cell Reprogramming to an Alternate Cell Fate|
|Status||This meeting has already occurred|
Adenoviral delivery of Pdx1/Ngn3/MafA (3TF) to the pancreas of immunocompromised, Rag1-/- mice reprograms pancreatic acinar cells into insulin-expressing beta-like cells. However,we have found that tetracycline-induced acinar-specific expression of 3TF transgene in Rag1+/+ mice does not result in the production of new insulin-secreting cells. Instead, 7 days of 3TF transgene expression results in the conversion of acinar cells into progenitor, duct-like cells and is associated with a potent inflammatory response with the pancreata exhibiting hallmarks of acinar-to-ductal metaplasia (ADM). We have found that when macrophages are depleted prior to and during inducing 3TF expression, the ADM-like phenotype does not occur, and acinar-derived beta-cells are generated.