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GFR will be measured in conscious mice based on the decay rate
of plasma FITC-inulin following a single bolus intravenous
injection of FITC-inulin. This method does not require urine
collection, and GFR can be periodically measured
in same mouse.
Additional approach for determining GFR in conscious mouse is
based on creatinine clearance rate. Mouse 24-hour urine will be
collected using metabolic cage. Plasma and urinary creatinine
concentration will be determined using HPLC
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Released July 13, 2020