We have detected that you are using some form of ad-blocking or filtering.
Please consider white-listing Labnodes since 1) ad-blockers like uBlock break Labnodes functionality and 2) Labnodes does not serve ads.
Tissue specific glucose uptake is assessed by measuring the tissue specific uptake of [2- 3H]-deoxyglucose([2-3H]DG). [2-3H]DG is infused (0.2 µCi/min) for 40 minutes or injected (12 µCi). Arterial plasma samples are taken to determine the time course of [2-3H]DG during the 40 min period. [2-3H]DG is transported into cells and phosphorylated to yield [2-3H]DG-6-phosphate which is trapped in muscle. After 40 min mice are anesthetized with an intravenous infusion of pentobarbital and tissues of interest are rapidly removed and frozen in liquid nitrogen. This method has been applied during insulin- and exercise-stimulated conditions (Halseth et al. Am. J. Physiol. 1999).
© 2010-2022. All Rights Reserved to Vanderbilt University. Vanderbilt University is committed to principles of equal opportunity and affirmative action.
Released July 13, 2020