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Added on October 7, 2014 at 1:52 PM by Tripp, Fran
Modified on October 7, 2014 at 1:52 PM by Tripp, Fran
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MMPC: Metabolic Regulation Core List of Services Master

Tissue specific glucose uptake is assessed by measuring the tissue specific uptake of [2- 3H]-deoxyglucose([2-3H]DG). [2-3H]DG is infused (0.2 µCi/min) for 40 minutes or injected (12 µCi). Arterial plasma samples are taken to determine the time course of [2-3H]DG during the 40 min period. [2-3H]DG is transported into cells and phosphorylated to yield [2-3H]DG-6-phosphate which is trapped in muscle. After 40 min mice are anesthetized with an intravenous infusion of pentobarbital and tissues of interest are rapidly removed and frozen in liquid nitrogen. This method has been applied during insulin- and exercise-stimulated conditions (Halseth et al. Am. J. Physiol. 1999).