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Amino Acid Kinetics

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Added on October 7, 2014 at 1:51 PM by Tripp, Fran
Modified on October 7, 2014 at 1:51 PM by Tripp, Fran
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The turnover of phenyalanine (3H ring 2,3,4,5,6 phenylalanine) , glutamine (U-14C-glutamine) and leucine (1-14C-leucine) is assessed by a primed continuous infusion of their respective isotopes for 2 hours (0.2-0.4 µCi/min). Blood samples (20 µl) are taken after a steady state is reached to assess plasma amino acid specific activity. Blood samples are mixed with an equal volume of 6% sulfosalicylic acid. Incorporation of tracer in tissue protein is used to assess tissue specific protein synthesis.