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Added on October 7, 2014 at 1:48 PM by Tripp, Fran
Modified on October 7, 2014 at 1:48 PM by Tripp, Fran
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MMPC: Metabolic Regulation Core List of Services Master

The contribution of gluconeogenesis to the rate of glucose appearance is estimated from the specific activities of 14C-labeled hepatic uridine diphosphoglucose (this is assumed to reflect the specific activity of hepatic glucose 6-phosphate), and hepatic phosphoenolpyruvate (PEP) following the infusion of [U-14C]lactate. [3-3H]glucose is used to measure the rate of glucose appearance. Gluconeogenesis is equal to the rate of glucose appearance x [14C]UDP-glucose specific activity/[14C]PEP specific activity x 2. Glycogenolysis is equal to the difference between rates of glucose appearance and gluconeogenesis. The contribution of the kidney to these measurements is assumed to be small.