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Mice are sacrificed and flushed with 30 ml saline. The heart with ascending aorta is embedded and snap-frozen in liquid N2. Cryosections of 10 um thickness is taken from the region of the proximal aorta. Cryosections are stained with Oil Red O and counterstained with hematoxylin. In addition to the aortic cross-sections, whole aortas will be analyzed in "en face" preparations to evaluate the distribution and characteristics of atherosclerotic lesions in the distal aorta. After the removal of the heart and the aortic arch, the entire remainder of the thoracic and abdominal aorta is dissected from the carcass. An incision is then performed longitudinally and for the total length of the specimen, so to expose the inside face. The open face aorta is pinned out on a black cardboard submerged in saline, and then stained with Oil Red O.
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Released July 13, 2020