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Total lipids are extracted and lipid classes separated by TLC as described above. Lipid ester spots are scraped from the plates and methylated. Fatty acids of lipid esters can be methylated without removal of the lipid from the silica gel. However, in some applications, we have found it advantageous to elute the lipid from the silica gel prior to methylation. The fatty acid profile of the lipid class is also determined. By this method total lipid mass and fatty acid profile for each lipid is determined.
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Released July 13, 2020