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Lipids are extracted from tissue, cells, or plasma. Individual lipid classes are separated by thin layer chromatography. Lipid classes are visualized by either iodine vapors or rhodamine 6G, scraped from the plates and eluted from the silica gel. For colorimetric analysis of lipid classes, the individual lipids are eluted from the TLC silica gel. Phospholipids are analyzed either in total lipid extract or on phospholipid fraction eluted from TLC plates. Cholesterol esters and unesterified cholesterol are analyzed by the method of Babson. Alternatively, cholesterol is analyzed by gas chromatography. Lipid classes containing fatty acids are also quantitated by gas chromatography.
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Released July 13, 2020