We have detected that you are using some form of ad-blocking or filtering.
Please consider white-listing Labnodes since 1) ad-blockers like uBlock break Labnodes functionality and 2) Labnodes does not serve ads.
Plasma short chain fatty acids are analyzed by the following procedure: to 200 ul of EDTA plasma in a 1.5 ml Eppendorf microfuge tube is added 20 µl of internal standard and 1 ml of absolute ethanol. The sample is mixed thoroughly, centrifuged, and the supernatant is recovered. The sample is evaporated using a Speed Vac and dissolved in 15 µl water, and prior to injection 5 µl of orthophosphoric acid (25%) is added. The short chain fatty acids are separated on a 6' x 2 mm glass column packed with SP-1200/1%H3PO4 on 80/100 Chromosorb W AW.
© 2010-2021. All Rights Reserved to Vanderbilt University. Vanderbilt University is committed to principles of equal opportunity and affirmative action.
Released July 13, 2020