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Note: This plasmid was published with different name called pMCS.66.2272.

This is a basal exchange vector for RMCE containing heterotypic LoxP sites. A lox71 site, flanked by Sac I and Not I, and a lox2272 site, flanked by Xho I and Kpn I, were inserted into the same sites of pBluescript KS+. Oligonucleotide pairs containing a lox71 site, flanked by Sac I and Not I, and a lox2272 site, flanked by Xho I and Kpn I, were synthesized, kinased, annealed, and ligated into the same sites of pBluescript KS+.

Oligonucleotide sequences (lox sites are indicated in upper case):

  • lox66.Sac/Not1 5’-cATAACTTCGTATAATGTATGCTATACGAACGGTAgc-3’
  • lox66 Sac/Not2 5’-ggccgcTACCGTTCGTATAGCATACATTATACGAAGTTATgagct-3’
  • lox2272 Xho/Kpn1 5’-tcgagATAACTTCGTATAAAGTATCCTATACGAAGTTATggtac-3’
  • lox2272 Xho/Kpn2 5’-cATAACTTCGTATAGGATACTTTATACGAAGTTATc

 

Keywords: RMCE Lox2272 Lox71

Category
Plasmid
Record History
Added on December 23, 2008 at 9:59 AM by Gangula, Rama
Modified on September 5, 2013 at 12:44 PM by Magnuson, Mark
Shared with (contributions)
Public: Shared Collection

Vector Annotations

Vector Map J1AL73Dy.png
Genbank File pMCS.712272.gb
Backbone Vector pBluescript KS+
Construct Size 3,055 bp (approximate)
Bacterial Stock No
Storage Temperature -20°C
Stock Concentration 0.1 µg/µL
Addgene 22732

Source

Laboratory Mark Magnuson
Made byKathy Shelton
Stock Date December 23, 2008

Inventory Location

Private

Publications / Citations

  1. Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice. Chen SX, Osipovich AB, Ustione A, Potter LA, Hipkens S, Gangula R, Yuan W, Piston DW, Magnuson MA (2011) Dis Model Mech 4(4): 537-47
    › Primary publication · 21324933 (PubMed) · PMC3124063 (PubMed Central) · Added on 11/8/2013

MeSH Terms

Alleles Animals Chromosomes, Artificial, Bacterial Embryo, Mammalian Embryonic Stem Cells Fluorescence Genetic Loci Genetic Vectors Luminescent Proteins Mice Mutagenesis, Insertional Organ Specificity Proteins Rabbits Recombinases RNA, Untranslated

Attachment

Document bsl66.2272.pdf - Added on September 25, 2009 at 12:00 AM by Rama Gangula