Kidney Services - GFR - FITC-inulin; HPLC Cr


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Added on August 5, 2014 at 10:46 AM by Tripp, Fran
Modified on August 5, 2014 at 10:46 AM by Tripp, Fran
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MMPC: CPCC Services Master

GFR – FITC-Inulin; HPLC Cr GFR will be measured in conscious mice based on the decay rate of plasma FITC-inulin following a single bolus intravenous injection of FITC-inulin. This method does not require urine collection, and GFR can be periodically measured in same mouse. Additional approach for determining GFR in conscious mouse is based on creatinine clearance rate. Mouse 24-hour urine will be collected using metabolic cage. Plasma and urinary creatinine concentration will be determined using HPLC approach.