Vascular Morphology A variety of tissues (heart, aorta, kidney, brain) can be processed in four µm sections. Aortic sections are examined for wall thickening, perivascular fibrosis, and fibrin deposition. The inner border, the lumen outer border, the tunica media are traced in each arterial image with Masson's trichrome stain and imaged at a magnification of 200X. The lumen ratio (the medial thickness to internal diameter and the area fibrosis (collagen deposition stained with aniline blue) surrounding blood vessels are calculated and compared. Perivascular fibrosis is determined as the ratio of the area of fibrosis surrounding the vessel wall to the total vessel area.
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Released April 5, 2018