In vitro Morphology, Morphometrics and Histology


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Added on August 5, 2014 at 10:10 AM by Tripp, Fran
Modified on August 5, 2014 at 10:58 AM by Tripp, Fran
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MMPC: CPCC Services Master

In vitro Morphology, Morphometrics and Histology
A limited necropsy is conducted, noting gross observations, and removing and weighing the heart and lungs separately. After fixation, the heart is sectioned in a standard four-chamber view. Digital photographs on each heart is recorded and archived in a web-accessible format. Chamber and mural dimensions will be measured. The fixed hearts will be maintained in a physical archive, while one slice will be paraffin embedded. 4-5 sections will be cut and prepared for H& E and Masson trichrome. Digital photomicrographs will be recorded and archived together with summary evaluations. A cardiologist with special expertise in mouse cardiac development and histology reviews all gross and microscopic sections.