Frequently Asked Questions - MMPC
Q. At what age should I ship mice for clamping
A. We prefer mice to be shipped to us at 7 wks of age. Mice will
be released from Vandy quarantine by 13-15 wks and undergo surgery
at that time.
Q. I am performing nutrition studies. Can the MMPC use
my custom diet?
A. Yes! Please contact us at least 2 wks before shipping.
Q. Breeding can be unpredictable and slow. Can we send
mice as they become available or do we have to send them as a
complete cohort at all one time?
A. You can send them all at once or as they become available,
whatever is most convenient for you. Note however multiple
shipments is not recommended. It increases costs (shipping and
serology) and will add variability to the data.
Q. Can I combine your "Non-Invasive Special" with one of
your "Clamp Specials"?
- Yes! We can make non-invasive measurements and perform a clamp
all in the same mouse.
- You describe different types of clamps. How do I know
which one to request?
A. To assess (a) insulin sensitivity order a hyperinsulinemic
euglycemic clamp; (b) response to low blood glucose order a
hyperinsulinemic, hypoglycemic clamp; and (c ) beta cell insulin
secretion order a hyperglycemic clamp.
- If I send you my mice do I retain control of the
distribution of the mouse and the data generated by the
A. Yes. Please see our Materials Transfer Agreement (http://www.mmpc.org/MTA.pdf
- Am I required to include Vanderbilt MMPC faculty and
staff as authors on papers including data generated by the
- No. Vanderbilt MMPC operates on a fee for service basis.
Authorship for MMPC faculty and staff is at the discretion of the
Frequently Asked Questions – Energy Balance
Vanderbilt MMPC Energy Balance Core: Considerations
Before Starting a Study
- The minimum number of animals
recommended per group/treatment/diet/genotype is
- Unless your research question is specifically gender related
all animals should be the same sex due to
known effects of gender on energy balance.
- At the time of testing animals should be fully mature (8
weeks+) and age matched as much as possible (±2 weeks). It is
recommended that all animals be sent in a single shipment.
- Current Vanderbilt quarantine for animals shipped from external
institutions is approximately 6 weeks.
- When comparing between genotypes (particularly on mixed genetic
backgrounds) wild-type littermates should be used as controls.
- Studies can be performed with animals on special diets but
these diets must be received a minimum of 2 weeks prior to the
start of the study. If the animals need to be on the diet while in
quarantine; fenbendazole must be added to the diet. If fenbendazole
is contraindicated for your mice, contact the core director.
- Measurement of body composition is included in all indirect
- Before starting experiments we need to know the genotype,
genetic modification and background strain of the animals. This is
required so we can make sure that the experiments are run
correctly. **Please contact the core director for more
- On completion of the study ear punches will be returned to the
investigator for validation of genotypes.