The microinjection of mouse ESCs into 3.5 day old mouse
embryos is performed to generate chimeric mice from
genetically modified mESCs. This method is an essential step
in the generation of precisely modified alleles (e.g. conditional,
knock-in or null alleles). The ESC microinjection experiments
are generally performed using 3.5 day old embryo derived
from natural matings of C57Bl/6 mice. Pups will be born about
3 weeks after injected blastocyst are transferred to a recipient
animal. Once hair color has established, percentage of chimericism
is noted.
Please note that after the microinjected embryos have been transferred into the uterus of a pseudopregnant recipient animal, the animals become the financial responsibility of the investigator and are housed under his/her animal protocol number within the Vanderbilt Barrier facility.
blast_injections2.wmv - Added on July 30, 2010 at 2:57 PM by Jill Lindner
Microinjection of mESCs into a mouse blastocyst using an XY clone laser
ES_Cell_injection.jpg - Added on March 29, 2016 at 1:46 PM by Jennifer Skelton
microinjection of mESC into blastocysts