This form may be used to request injection of DNA into the pronucleus of one cell mouse embryos.


Category
Service
Record History
Added on June 14, 2010 at 10:12 AM by Lindner, Jill
Modified on October 4, 2017 at 2:09 PM by Skelton, Jennifer
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TMESCSR: Service Forms Master

This service provides an efficient means of introduction foreign DNA, usually plasmid or BAC derived fusion genes, into the mouse genome. DNA is microinjected into the pronucleus of 0.5 day fertilized mouse embryos. Surviving embryos are transferred to the oviduct of a pseudopregnant recipient and the animals are housed under the protocol number of the investigator but husbandry is provided by the TMESCSR and DAC. The gestation period of the mouse is 19 to 21 days. Pups are born, and will be weaned, tailed and numbered by core personnel at 3-4 weeks of age. The investigator will need to perform DNA isolation and analyze the DNA by PCR or Southern blotting to identify transgenic founders.

The following data may be useful in estimating the number of injection days required to generate transgenic founders.  Typically, ~100 embryos are injected per "injection day".  

TMESCR Pronuclear Injection Statistics (Oct. 2008 - December 2015)

DNA type

Embryonic Strain

# constructs

injected

Total # transgenic pups

% Transgenic pups /

total born

Plasmid or BAC

B6D2

91

271

16%

Plasmid or BAC

C57BL/6

55

126

15%

 

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Attachment

Document Pronuclear_DNA_Form_1017.docx - Added on October 4, 2017 at 2:09 PM by Jennifer Skelton