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This page describes the policy and procedures for tailing, numbering, and re-tailing mice.


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Added on April 12, 2010 at 3:55 PM by Skelton, Jennifer
Modified on November 3, 2016 at 3:09 PM by Skelton, Jennifer
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TMESCSR: Policies Master

Tailing and Ear Punching:

  1. At 3 weeks of age, mice will be ear punched and tailed by the TMESCSR staff.
  2. The TMESCSR staff will e-mail the contact person for the project and the investigator to let them know when the tail samples are ready for pick-up.
  3. Within the following 3 weeks, the investigator must let the TMESCSR staff know which animals are positive for the gene of interest.
  4. The TMESCSR will submit a transfer request to move the transgenic animals to the investigator's room for housing.
  5. Pending approval of the serology results by the DAC staff, the transgenic founders will be moved to the investigator's room for housing and mating.

Retailing

If for whatever reason there is a failure to correctly genotype some or all of the pups after the TMESCSR provides tail samples to the investigator, the following applies:

  1. The investigator should contact the TMESCSR to confirm which animals need to be retailed and should list a reason as to why there was a failure to genotype.
  2. The TMESCSR staff will contact the DAC to get approval for the retailing procedure and complete a plan for which mice need to be retailed.
  3. Once approved, the TMESCSR staff will retail the appropriate animals and the investigator will be notified when the samples are ready to be picked up.
  4. The investigator is responsible for an hourly service fee for retailing.
  5. If the investigator cannot confirm which animals are correctly genotyped within three weeks of the animals being retailed, the animals will be sacrificed.
  6. If the investigator can correctly identify transgenic animals after retailing, a transfer request will be submitted by the TMESCSR to release the positive animals to the investigator's room for housing.