NOTICE -- Labnodes will be unavailable On Saturday evening to Sunday morning for scheduled maintenance. We appreciate your patience and we ask that you let us know if you experience any issues.
NOTICE: Labnodes now supports email addresses. If your email changed, please update your profile today.

This plasmid can be used to assemble a gene targeting vector for generating a conditional allele.  It contains two tandemly oriented loxP sites and two tandem FRT sites along with pgk-neo (positive) and pgk-tk (negative) selection cassettes in pBluescript KS+.  The pgk-neo and pgk-tk cassettes were obtained from the plasmid pNTK(A).  Unique restriction enzyme cloning sites are indicated in red. 

Record History
Added on February 9, 2009 at 1:23 PM by Gangula, Rama
Modified on August 18, 2010 at 12:34 PM by Gangula, Rama
Shared with (contributions)
Public: Shared Collection

Vector Annotations

Vector Map fh3VLjdP.png
Genbank File
Backbone Vector pBluescript KS+
Construct Size 7,012 bp (approximate)
Bacterial Stock No
Storage Temperature -20°C
Stock Concentration 0.1 µg/µL
Addgene Not Provided


Laboratory Mark Magnuson
Made byKathy Shelton
Stock Date February 9, 2009

Inventory Location



Document frtloxp.pdf - Added on October 2, 2009 at 12:00 AM by Rama Gangula