Common Name | gklox |
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Research Applications |
Cre-lox system |
MMRRC ID | 011949-UNC |
Jackson Laboratories Stock No | Not provided |
VCMR ID | Not provided |
Additional Strain Information | Not provided |
Mutation #1: Targeted Mutagenesis | |||
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Type of Allele | Conditional Null | ||
Targeted Gene | Name: Glucokinase Symbol: Gck NCBI: 103988 | ||
Allele |
Name: targeted mutation 1.1 Symbol: Gcktm1.1Mgn MGI: MGI:2177709 |
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Description of Targeting Vector | A gene targeting strategy was used to flank exons 9 and 10 in the glucokinase gene with two tandomly-oriented loxP sites. This strain allows for the tissue specific knock-out of glucokinase to be made. For example, crossing the gklox/lox mice with an insulin-cre transgenic mouse generates a beta cell specific knock-out of glucokinase. Genotype by DNA PCR using primers 5'-TGT CTC AAT TTG CTG TGT CCT CCA-3' and 5'-TCT GTT AAT GCA AAT GCT CGT GTT-3'. A 710 bp band will be amplified for the gklox allele and a 605 bp band for the wild type allele. Homozygous gklox/lox mice are viable but have a blood glucose concentrations slightly higher than wild types (194 +/- 3 mg/dl vs. 175 +/- 8 mg/dl). This finding suggests that the insertion of a loxP site (and some flanking sequences) between exons 8 and 9 may have caused a slight attenuation in glucokinase gene expression compared to mice with two wild type alleles. |
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Vector Genbank File |
pBOB.gb | ||
Allele Map | Not Provided | ||
PCR Genotyping Protocol | Not provided | ||
Citations |
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Strain Type | Congenic Strain |
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Chimera/Founder Genetic Background | 129S6/SvEvTac |
Current Genetic Background | C57BL/6J |
Number of Generations Backcrossed | 10 |
Strain Description | After achieving germline transmission mice carrying the gklox allele were backcrossed for ten generations into a C57Bl/6J background. |
gk_g__w__pcr_protocol.doc - Added on July 27, 2010 at 9:52 AM by Jill Lindner
PCR protocol for genotyping mice.