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This plasmid contains both the mouse albumin promoter/enhancer sequence and coding sequences of the human growth hormone gene flanking a unique BamHI site. This plasmid may be used to assemble transgenes for expression in liver.  Note: The albumin promoter/enhancer sequence was originally obtained from Richard Palmiter.


Category
Plasmid
Record History
Added on December 22, 2008 at 3:22 PM by Gangula, Rama
Modified on May 1, 2013 at 11:28 AM by Magnuson, Mark
Shared with (contributions)
Public: Shared Collection

Vector Annotations

Vector Map GaEBLjzR.png
Genbank File pAlb.GH.gb
Backbone Vector pBluescript KS+
Construct Size 7,442 bp (approximate)
Bacterial Stock No
Storage Temperature -20°C
Stock Concentration 0.1 µg/µL
Addgene Not Provided

Source

Laboratory Mark Magnuson
Made byKathy Shelton
Stock Date December 22, 2008

Inventory Location

Private

Publications / Citations

  1. Dual roles for glucokinase in glucose homeostasis as determined by liver and pancreatic beta cell-specific gene knock-outs using Cre recombinase. Postic C, Shiota M, Niswender KD, Jetton TL, Chen Y, Moates JM, Shelton KD, Lindner J, Cherrington AD, Magnuson MA (1999) J Biol Chem 274(1): 305-15
    › Primary publication · 9867845 (PubMed) · Added on 1/31/2014

MeSH Terms

Albumins Alleles Animals Base Sequence DNA Primers Gene Deletion Glucokinase Glucose Homeostasis Insulin Integrases Islets of Langerhans Liver Mice Mice, Knockout Transgenes Viral Proteins