This was designed to be used to retrieve DNA sequences after recombineering of a BAC clone.  The plasmid contains a diphtheria toxin selection gene, DT-A, cloned between the Kpn I and Sal I restriction enzyme sites of pBluescript KS+.  This will place it outside of a homology arm in a gene targeting vector, thereby eliminating the need to use a HSV-thymidine kinase gene and gancyclovir in a gene targeting experiment.

This plasmid was previously call pBS.DT-A and renamed when submitted to AddGene.


Category
Plasmid
Record History
Added on December 23, 2008 at 11:05 AM by Gangula, Rama
Modified on September 5, 2013 at 12:50 PM by Magnuson, Mark
Shared with (contributions)
Public: Shared Collection

Vector Annotations

Vector Map vaJlNW3l.png
Genbank File pmcs.dta.gb
Backbone Vector pBluescript KS+
Construct Size 4,064 bp (approximate)
Bacterial Stock No
Storage Temperature -20°C
Stock Concentration 0.1 µg/µL
Addgene 22730

Source

Laboratory Mark Magnuson
Made byQiaoming Long
Stock Date December 23, 2008

Inventory Location

Private

Publications / Citations

  1. Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice. Chen SX, Osipovich AB, Ustione A, Potter LA, Hipkens S, Gangula R, Yuan W, Piston DW, Magnuson MA (2011) Dis Model Mech 4(4): 537-47
    › Primary publication · 21324933 (PubMed) · PMC3124063 (PubMed Central) · Added on 11/8/2013

MeSH Terms

Alleles Animals Chromosomes, Artificial, Bacterial Embryo, Mammalian Embryonic Stem Cells Fluorescence Genetic Loci Genetic Vectors Luminescent Proteins Mice Mutagenesis, Insertional Organ Specificity Proteins Rabbits Recombinases RNA, Untranslated