|Jackson Laboratories Stock No||Not provided|
|Additional Strain Information||Not provided|
|Mutation #1: RMCE Targeted Mutagenesis|
|Type of Allele||Cassette Acceptor|
|Targeted Gene||Name: gene trap ROSA 26, Philippe Soriano|
Name: targeted mutation 1
|Description of Targeting Vector||
This mouse line contains a loxed cassette acceptor (LCA) allele in which a 5.17 kb region of the gene has been replaced by a lox71 site, a puromycin-(delta)-thymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter, a kanamycin resistance gene driven by the bacterial EM7 promoter, and a lox2272 site. These features enable use of Recombinase-Mediated Cassette Exchange (RMCE) for the rapid insertion of various DNAs into the the Rosa26 gene locus.
|Vector Genbank File||
|Allele Map||Not Provided|
|PCR Genotyping Protocol||Not provided|
|Type of Allele||Gene Replacement|
|Exchanged Cassette Gene Name|| ()
|Exchanged Cassette Allele Name||pRosa.EN.Cherry.bGsplicepA.Neo
|Description of Exchange Vector||
|Exchanged Cassette Genbank File||
|PCR Genotyping Protocol||
|Chimera/Founder Genetic Background||129S6/SvEvTac|
|Current Genetic Background||C57BL/6J|
|Number of Generations Backcrossed||2|
This strain is of a mixed genetic background that is approximately 25% 129S6 and 75% C57BL6/J.
This figure shows how this line of mice was made. Coding sequences for a red (mCherry) flourescent protein gene were inserted into an exchange cassette that allowed RMCE into a ROSA26 [LCA] allele. In this manner, mCherry is constitutively expressed under control of the endogenous ROSA26 promoter. The exchange plasmid also contains a 51bp translational enhancer (5' leader sequence from Xenopus beta-globin), a Kozak sequence upstream of the start codon, and intronic an polyA sequences from the rabbit beta-globin gene that confer stability to the mRNA.