Common Name | Rosa26EN.Cherry.Neo |
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Research Applications |
Fluorescent proteins |
MMRRC ID | 036286-UCD |
Jackson Laboratories Stock No | Not provided |
VCMR ID | NI |
Additional Strain Information | Not provided |
Mutation #1: RMCE Targeted Mutagenesis | |||
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Type of Allele | Cassette Acceptor | ||
Targeted Gene | Name: gene trap ROSA 26, Philippe Soriano Symbol: Gt(ROSA)26Sor NCBI: 14910 | ||
Allele |
Name: targeted mutation 1 Symbol: Rosa26tm1(LCA) MGI: MGI:5085307 |
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Description of Targeting Vector | This mouse line contains a loxed cassette acceptor (LCA) allele in which a 5.17 kb region of the gene has been replaced by a lox71 site, a puromycin-(delta)-thymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter, a kanamycin resistance gene driven by the bacterial EM7 promoter, and a lox2272 site. These features enable use of Recombinase-Mediated Cassette Exchange (RMCE) for the rapid insertion of various DNAs into the the Rosa26 gene locus. |
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Vector Genbank File |
TargetVectorGenebankpRosa26.LCA.gb | ||
Allele Map | Not Provided | ||
PCR Genotyping Protocol | Not provided | ||
Type of Allele | Gene Replacement | ||
Exchanged Cassette Gene Name | ()
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Exchanged Cassette Allele Name | pRosa.EN.Cherry.bGsplicepA.Neo |
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Description of Exchange Vector | Not Provided |
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Exchanged Cassette Genbank File |
ExchangeVectorpRosa.EN.Cherry.bGsplicepA.neo.gb |
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PCR Genotyping Protocol |
Not provided |
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Citations |
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Strain Type | Mixed |
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Chimera/Founder Genetic Background | 129S6/SvEvTac |
Current Genetic Background | C57BL/6J |
Number of Generations Backcrossed | 2 |
Strain Description | This strain is of a mixed genetic background that is approximately 25% 129S6 and 75% C57BL6/J. |
LinearMapr26encherry.png - Added on July 12, 2011 at 10:52 AM by Jody Peters
This figure shows how this line of mice was made. Coding sequences for a red (mCherry) flourescent protein gene were inserted into an exchange cassette that allowed RMCE into a ROSA26 [LCA] allele. In this manner, mCherry is constitutively expressed under control of the endogenous ROSA26 promoter. The exchange plasmid also contains a 51bp translational enhancer (5' leader sequence from Xenopus beta-globin), a Kozak sequence upstream of the start codon, and intronic an polyA sequences from the rabbit beta-globin gene that confer stability to the mRNA.