Identification and characterization of RNA editing events within the 5-HT2C receptor.

Niswender CM, Sanders-Bush E, Emeson RB
Ann N Y Acad Sci. 1998 861: 38-48

PMID: 9928237 · DOI:10.1111/j.1749-6632.1998.tb10171.x

RNA editing is a post-transcriptional modification that generates an RNA transcript with a nucleotide sequence different from its gene. We have recently discovered RNA editing events, involving the conversion of adenosine bases to inosine residues, within the RNA encoding the serotonin 2C (5-HT2C) receptor. Editing events at four major positions, termed A, B, C and D, as well as one minor site termed C', are predicted to alter amino acids within the second intracellular loop of the G-protein coupled 5-HT2C receptor. Editing is mediated by at least two members of a family of adenosine deaminases and is contingent upon the presence of an extensive RNA duplex structure formed by exonic and intronic sequences of 5-HT2C receptor precursor messenger RNA (pre-mRNA). This critical secondary structure has been observed within brain pre-mRNA derived from four species; the isolation of edited 5-HT2C receptor transcripts from these samples further confirms the evolutionary conservation of this RNA processing event. Among members of the 5-HT2 receptor family, editing within second intracellular loop RNA is unique to the 5-HT2C receptor. Editing within the 5-HT2C receptor generates receptor isoforms that differ in their ability to interact with the phospholipase C signaling cascade in a transfected cell line, suggesting that this RNA processing event may contribute to the modulation of serotonergic neurotransmission in the central nervous system.

MeSH Terms (15)

Amino Acid Sequence Animals Base Sequence Brain Evolution, Molecular Humans Molecular Sequence Data Nucleic Acid Conformation Point Mutation Rats Receptor, Serotonin, 5-HT2C Receptors, Serotonin RNA Editing RNA Precursors Transcription, Genetic

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