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The cdr2(+) gene encodes a regulator of G2/M progression and cytokinesis in Schizosaccharomyces pombe.

Breeding CS, Hudson J, Balasubramanian MK, Hemmingsen SM, Young PG, Gould KL
Mol Biol Cell. 1998 9 (12): 3399-415

PMID: 9843577 · PMCID: PMC25645 · DOI:10.1091/mbc.9.12.3399

Schizosaccharomyces pombe cells respond to nutrient deprivation by altering G2/M cell size control. The G2/M transition is controlled by activation of the cyclin-dependent kinase Cdc2p. Cdc2p activation is regulated both positively and negatively. cdr2(+) was identified in a screen for regulators of mitotic control during nutrient deprivation. We have cloned cdr2(+) and have found that it encodes a putative serine-threonine protein kinase that is related to Saccharomyces cerevisiae Gin4p and S. pombe Cdr1p/Nim1p. cdr2(+) is not essential for viability, but cells lacking cdr2(+) are elongated relative to wild-type cells, spending a longer period of time in G2. Because of this property, upon nitrogen deprivation cdr2(+) mutants do not arrest in G1, but rather undergo another round of S phase and arrest in G2 from which they are able to enter a state of quiescence. Genetic evidence suggests that cdr2(+) acts as a mitotic inducer, functioning through wee1(+), and is also important for the completion of cytokinesis at 36 degrees C. Defects in cytokinesis are also generated by the overproduction of Cdr2p, but these defects are independent of wee1(+), suggesting that cdr2(+) encodes a second activity involved in cytokinesis.

MeSH Terms (17)

Amino Acid Sequence ATP-Binding Cassette Transporters Base Sequence Cell Division DNA, Fungal DNA Primers Fungal Proteins G2 Phase Gene Expression Genes, Fungal Mitosis Molecular Sequence Data Mutation Nitrogen Protein-Serine-Threonine Kinases Schizosaccharomyces Sequence Homology, Amino Acid

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