The alloreactive T cell response against the class Ib molecule H2-M3 is specific for high affinity peptides.

Dabhi VM, Hovik R, Van Kaer L, Lindahl KF
J Immunol. 1998 161 (10): 5171-8

PMID: 9820487

MHC class Ib molecule H2-M3 presents N-formylated peptides to CD8+ CTLs. Endogenous formylated peptides can come from the N-terminus of each of the 13 proteins encoded by the mitochondrial genome. In peptide competition assays, two of these peptides bind with high affinity, six bind with intermediate affinity, three bind with low affinity, and two do not bind measurably. Alloreactive CTLs from M3-specific, mixed lymphocyte cultures responded strongly against the two peptides with high affinity for M3, occasionally to peptides with intermediate affinity, and not at all to the rest. Long term lines and CTL clones reacted with only the high affinity peptides, demonstrating that alloreactive CTLs depend on specific peptides and that peptide affinity for class I correlates with alloantigenicity.

MeSH Terms (25)

Alleles Animals ATP-Binding Cassette Transporters ATP Binding Cassette Transporter, Subfamily B, Member 2 ATP Binding Cassette Transporter, Subfamily B, Member 3 Cell Line Chloramphenicol Clone Cells Cytotoxicity, Immunologic Epitopes, T-Lymphocyte Histocompatibility Antigens Class I Isoantigens Lymphocyte Culture Test, Mixed Mice Mice, Inbred BALB C Mice, Inbred C3H Mice, Inbred C57BL Mice, Inbred NZB Mitochondria N-Formylmethionine NADH Dehydrogenase Oligopeptides Protein Binding Protein Synthesis Inhibitors T-Lymphocytes, Cytotoxic

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