Determination of protein-protein interactions by matrix-assisted laser desorption/ionization mass spectrometry.

Farmer TB, Caprioli RM
J Mass Spectrom. 1998 33 (8): 697-704

PMID: 9786710 · DOI:10.1002/(SICI)1096-9888(199808)33:8<697::AID-JMS711>3.0.CO;2-H

A number of different procedures have been developed for use with matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS) for the analysis of non-covalent protein-protein complexes. These include use of specific matrix and laser combinations, accumulation of "first shot" spectra, modification of pH and solvent conditions during sample preparation and use of cross-linking agents to attach the monomers covalently to each other in the complex. The results have shown the techniques to be effective with some but not all complexes, although cross-linking is the most successful. The physical and chemical nature of the complex is critical and therefore a diversity of approaches is recommended for such studies.

MeSH Terms (9)

Animals Cattle Cross-Linking Reagents Humans Hydrogen-Ion Concentration Protein Conformation Proteins Specimen Handling Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

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