Increased endothelial cell expression of platelet-endothelial cell adhesion molecule-1 during hyperoxic lung injury.

Piedboeuf B, Gamache M, Frenette J, Horowitz S, Baldwin HS, Petrov P
Am J Respir Cell Mol Biol. 1998 19 (4): 543-53

PMID: 9761750 · DOI:10.1165/ajrcmb.19.4.2349

Lung injury is a frequent consequence of oxygen (O2) therapy administered to newborns and adults with respiratory distress. Acute exposure to hyperoxia results in a well-described pathophysiologic response in the lungs. Because inflammation is an important component of pulmonary O2 toxicity, we have an interest in identifying the inflammatory mediators that increase during hyperoxia. Platelet-endothelial cell adhesion molecule-1 (PECAM-1), a member of the immunoglobulin superfamily that is expressed at the junctions between endothelial cells, is essential to the transendothelial migration of leukocytes. We hypothesized that increased expression of PECAM-1 occurs in pulmonary endothelial cells during hyperoxic lung injury. Adult mice were exposed to 100% O2 for up to 96 h. We analyzed PECAM-1 expression by RNA blot hybridization, in situ hybridization, and immunohistochemistry. A increase in PECAM-1 mRNA was seen as soon as 2 d of hyperoxia relative to unexposed control mice. PECAM-1 mRNA and protein were found in endothelial cells of both large and small arteries. The expression of PECAM-1 in capillary vessels was further confirmed using in situ hybridization at the electron microscope level. This increase in PECAM-1 expression coincided with the appearance of leukocytes in lung tissue. These observations suggest that PECAM-1 expression is a relatively early step in the inflammation cascade, and intervention at this phase may be critical to the prevention of further damage.

MeSH Terms (17)

Alternative Splicing Animals Blotting, Western Endothelium Gene Expression Hyperoxia In Situ Hybridization Lung Male Mice Mice, Inbred C3H Microscopy, Immunoelectron Oxygen Platelet Endothelial Cell Adhesion Molecule-1 Pneumonia Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger

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