Fission yeast cdc24(+) encodes a novel replication factor required for chromosome integrity.

Gould KL, Burns CG, Feoktistova A, Hu CP, Pasion SG, Forsburg SL
Genetics. 1998 149 (3): 1221-33

PMID: 9649516 · PMCID: PMC1460225

A mutation within the Schizosaccharomyces pombe cdc24(+) gene was identified previously in a screen for cell division cycle mutants and the cdc24(+) gene was determined to be essential for S phase in this yeast. We have isolated the cdc24(+) gene by complementation of a new temperature-sensitive allele of the gene, cdc24-G1. The DNA sequence predicts the presence of an open reading frame punctuated by six introns which encodes a pioneer protein of 58 kD. A cdc24 null mutant was generated by homologous recombination. Haploid cells lacking cdc24(+) are inviable, indicating that cdc24(+) is an essential gene. The transcript of cdc24(+) is present at constant levels throughout the cell cycle. Cells lacking cdc24(+) function show a checkpoint-dependent arrest with a 2N DNA content, indicating a block late in S phase. Arrest is accompanied by a rapid loss of viability and chromosome breakage. An S. pombe homolog of the replicative DNA helicase DNA2 of S. cerevisiae suppresses cdc24. These results suggest that Cdc24p plays a role in the progression of normal DNA replication and is required to maintain genomic integrity.

MeSH Terms (21)

Alleles Amino Acid Sequence Base Sequence Cell Cycle Cell Cycle Proteins Cell Division Fungal Proteins Genetic Complementation Test Genotype Guanine Nucleotide Exchange Factors Introns Molecular Sequence Data Proto-Oncogene Proteins Recombination, Genetic Repressor Proteins Restriction Mapping Saccharomyces cerevisiae Proteins Schizosaccharomyces Sequence Alignment Sequence Homology, Amino Acid Temperature

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