Brain creatine kinase with aging in F-344 rats: analysis by saturation transfer magnetic resonance spectroscopy.

Smith CD, Landrum W, Carney JM, Landfield PW, Avison MJ
Neurobiol Aging. 1997 18 (6): 617-22

PMID: 9461059 · DOI:10.1016/s0197-4580(97)00156-5

We measured in vivo forward flux of the creatine kinase reaction in rat forebrain in young (Y: 6 month, n = 13), mid-aged (M: 12 month, n = 7) and aged (O: 27 month, n = 10) animals using 31P magnetic resonance saturation transfer. Forward flux was reduced in the aged rats (Y: 0.42 +/- 0.08; M: 0.41 +/- 0.10; O: 0.31 +/- 0.03 s(-1) +/- SD; p = 0.008 O vs. Y). In vitro studies in a subset of the same rats showed a parallel decline in CK activity (Y: 2.16 +/- 0.40; M: 2.17 +/- 0.25; O: 1.56 +/- 0.06 IU +/- S.D.; p = 0.002 O vs. Y). The in vivo spectroscopic and in vitro biochemical measures were significantly correlated. Reduced creatine kinase activity could account for the observed decreased forward flux in aging brain. Intracellular pH, phosphocreatine/inorganic phosphate ratio, and phospocreatine/gamma-adenosine triphosphate ratio did not differ between groups. Forward flux may represent a better measure of brain energy function than relative phosphocreatine or adenosine triphosphate levels observable in vivo.

MeSH Terms (9)

Aging Animals Brain Creatine Kinase Kinetics Male Nuclear Magnetic Resonance, Biomolecular Rats Rats, Inbred F344

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