Adenovirus-mediated gene transfer during initial organogenesis in the mammalian embryo is promoter-dependent and tissue-specific.

Baldwin HS, Mickanin C, Buck C
Gene Ther. 1997 4 (11): 1142-9

PMID: 9425436 · DOI:10.1038/sj.gt.3300525

Replication-defective adenoviruses have received increasing attention as vectors for exogenous gene administration in a variety of experimental and pathological conditions. However, little information exists about their utility for in utero gene therapy, and no information exists concerning their efficacy for gene delivery during initial organogenesis in the mammalian embryo. To evaluate the feasibility of using these vectors for exogenous gene transduction during the initial stages of organogenesis in the mammal, we injected an adenovirus vector carrying the bacterial beta-galactosidase (lacZ) gene under the control of either the cytomegalovirus (CMV) promoter or the Rous sarcoma virus (RSV) long terminal repeat (LTR) into early, post-gastrulation, mouse embryos, and evaluated expression following 36-48 h in culture. These studies suggest that adenovirus-mediated gene delivery may provide an efficient method of gene transduction during critical developmental stages with no detectable adverse effects on normal development during early morphogenesis. In addition, the type of promoter used had a significant effect on the tissue distribution of gene expression.

MeSH Terms (17)

Adenoviridae Animals Avian Sarcoma Viruses Cells, Cultured Cytomegalovirus Embryo, Mammalian Endocardium Endothelium, Vascular Gene Expression Genetic Vectors Gene Transfer Techniques Lac Operon Mice Mice, Inbred ICR Morphogenesis Promoter Regions, Genetic Repetitive Sequences, Nucleic Acid

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