Expression of drug-metabolizing enzymes.

Guengerich FP, Parikh A
Curr Opin Biotechnol. 1997 8 (5): 623-8

PMID: 9353222 · DOI:10.1016/s0958-1669(97)80039-0

A principal advance in the production of drug-metabolizing enzymes has been the development of catalytically self-sufficient cytochrome P450 systems, including additional P450-reductase fusion proteins and Escherichia coli and baculovirus coexpression constructs. Continuing work with glutathione transferases has resulted in the identification of important residues by random mutagenesis screening techniques, as well as in the engineering of model Salmonella typhimurium strains for genotoxicity analysis.

MeSH Terms (14)

Animals Cell Line Cloning, Molecular Cytochrome P-450 Enzyme System Escherichia coli Genetic Engineering Mutagenesis Mutagenicity Tests NADPH-Ferrihemoprotein Reductase Pharmaceutical Preparations Recombinant Fusion Proteins Recombinant Proteins Salmonella typhimurium Transfection

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