Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization.

Link AJ, Phillips D, Church GM
J Bacteriol. 1997 179 (20): 6228-37

PMID: 9335267 · PMCID: PMC179534 · DOI:10.1128/jb.179.20.6228-6237.1997

MeSH Terms (14)

Alleles Bacterial Proteins Cloning, Molecular Electroporation Escherichia coli Escherichia coli Proteins Gene Deletion Genetic Engineering Genetic Vectors Genome, Bacterial Mutagenesis, Insertional Open Reading Frames Phenotype Polymerase Chain Reaction

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