The HIV-1 Nef protein acts as a connector with sorting pathways in the Golgi and at the plasma membrane.

Mangasarian A, Foti M, Aiken C, Chin D, Carpentier JL, Trono D
Immunity. 1997 6 (1): 67-77

PMID: 9052838 · DOI:10.1016/s1074-7613(00)80243-5

The HIV Nef protein down-regulates the cell surface expression of CD4 and of MHC I at least in part through accelerated endocytosis. To investigate further the mechanism of this effect, we created chimeric integral membrane proteins comprising the extracellular and transmembrane regions of CD4 or CD8 and Nef as the cytoplasmic domain. These fusion molecules could down-modulate CD4 in trans in a dileucine-dependent manner. Furthermore, in spite of lacking receptor-derived internalization signals, the Nef-containing chimeras underwent both Golgi retention and rapid endocytosis via clathrin-coated pits. Taken together, these data suggest that Nef down-regulates CD4 and probably MHC I by physically connecting these receptors with sorting pathways in the Golgi and at the plasma membrane.

MeSH Terms (15)

CD4 Antigens CD8 Antigens Cell Compartmentation Cell Membrane Coated Pits, Cell-Membrane Cytoplasm Down-Regulation Endocytosis Gene Products, nef Golgi Apparatus HIV-1 Humans Microscopy, Confocal nef Gene Products, Human Immunodeficiency Virus Recombinant Fusion Proteins

Connections (1)

This publication is referenced by other Labnodes entities: