Induction of human cytotoxic T lymphocytes specific for prostate-specific antigen.

Xue BH, Zhang Y, Sosman JA, Peace DJ
Prostate. 1997 30 (2): 73-8

PMID: 9051144 · DOI:10.1002/(sici)1097-0045(19970201)30:2<73::aid-pros1>3.0.co;2-l

Prostate-specific antigen (PSA), a tissue-specific protein expressed by most adenocarcinomas of the prostate, might be a useful target for T-cell-mediated immunotherapy of prostate cancers. The current study examined whether it is possible to elicit human cytotoxic T lymphocytes (CTL) with specificity for PSA. A synthetic nonamer peptide, corresponding to residues 146-154 of PSA and containing a canonical HLA-A2-binding motif, was shown to stabilize the expression of HLA-A2 on the T2 antigen-processing mutant cell line. Repeated in vitro stimulation of peripheral blood lymphocytes from a normal HLA-A2+ donor induced CTL with specificity for the PSA 146-154 peptide. The peptide-induced CTL expressed the CD4- CD8+ cell surface phenotype and were restricted by HLA-A2. A large portion of patients with prostate cancer express the HLA-A2 phenotype, implying that many prostate cancers might be targeted by HLA-A2-restricted CTL with specificity for the PSA 146-154 epitope.

MeSH Terms (14)

Adenocarcinoma Epitopes HLA-A2 Antigen Humans Immunity, Cellular Immunization Immunotherapy Male Peptide Fragments Peptides Prostate-Specific Antigen Prostatic Neoplasms T-Lymphocytes, Cytotoxic Tumor Cells, Cultured

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