Prednisone increases PGH-synthase 2 in atopic humans in vivo.

Dworski RT, Funk CD, Oates JA, Sheller JR
Am J Respir Crit Care Med. 1997 155 (1): 351-7

PMID: 9001335 · DOI:10.1164/ajrccm.155.1.9001335

We compared the expression of prostaglandin H synthase-2 (PGHS-2, cyclooxygenase) message and protein in alveolar macrophages (AM) and blood monocytes (BM) from 20 atopic subjects (AS) and nine control subjects (CS) at baseline and after 1 to 14 d of oral administration of therapeutic doses of prednisone. At baseline, the amounts of PGHS-2 mRNA in AM and BM varied within a similar range among subjects from each group. PGHS-2 protein was present in AM and BM from most AS, but it was also present in CS. In AS, PGHS-2 mRNA and protein significantly increased after prednisone administration, whereas in CS PGHS-2 message and protein remained undetectable or, if present at baseline, was decreased after prednisone. Ex vivo both atopic and normal monocytes and macrophages exhibited the expected decrease in stimulated PGHS-2 mRNA with glucocorticoids. PGHS-1 expression was not altered by prednisone in either group. The differential regulation of the inducible PGHS isoform by prednisone may be mediated by effects on cytokine production in AS.

MeSH Terms (14)

Adult Anti-Inflammatory Agents Blotting, Northern Blotting, Western Female Humans Hypersensitivity, Immediate Macrophages, Alveolar Male Middle Aged Monocytes Polymerase Chain Reaction Prednisone Prostaglandin-Endoperoxide Synthases

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