IL-15 can substitute for the marrow microenvironment in the differentiation of natural killer cells.

Puzanov IJ, Bennett M, Kumar V
J Immunol. 1996 157 (10): 4282-5

PMID: 8906800

NK cells require an intact bone marrow microenvironment to acquire lytic function. In mice rendered osteopetrotic by 17beta-estradiol treatment, NK1.1 positive cells are arrested in a nonlytic state. Culture with as little as 2 ng/ml of murine IL-15 (mIL-15), a cytokine produced by macrophages and stromal cells, causes these immature NK1.1+ cells to acquire lytic activity. By contrast, approximately 10- to 50-fold greater amount of mIL-2 was required to induce similar level of cytotoxicity. After culture with mIL-15, the relatively low expression of B220, CD11b, and Ly-49 molecules on immature NK1.1+ cells was increased to levels comparable to those of mature splenic NK1.1+ cells. mIL-15 also caused a greater expansion of NK1.1+CD3- cells as compared with NK1.1+CD3+ cells. We conclude that IL-15 is a specific maturation factor for NK cells and that it can mimic the marrow microenvironment in vitro.

MeSH Terms (20)

Animals Antigens, Ly Antigens, Surface Bone Marrow Bone Marrow Cells Cell Differentiation Cells, Cultured Female Interleukin-2 Interleukin-15 Killer Cells, Natural Lectins, C-Type Leukocyte Common Antigens Macrophage-1 Antigen Male Membrane Glycoproteins Mice Mice, Inbred C57BL Mice, Inbred DBA Receptors, NK Cell Lectin-Like

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