Helicobacter pylori cytotoxin induces vacuolation of primary human mucosal epithelial cells.

Harris PR, Cover TL, Crowe DR, Orenstein JM, Graham MF, Blaser MJ, Smith PD
Infect Immun. 1996 64 (11): 4867-71

PMID: 8890255 · PMCID: PMC174461

We investigated whether Helicobacter pylori cytotoxin induces vacuolation in primary epithelial cells from normal human mucosa. Epithelial cells purified by enzyme digestion and elutriation were evaluated for vacuolation in a blinded protocol by light and electron microscopy before and after incubation with culture supernatant (CS) from H. pylori 60190, which has vacuolating activity for HeLa cells (Tox+), and isogenic H. pylori mutant 60190-v1, which lacks this activity (Tox-). Primary epithelial cells (>98% pure) exposed to CS from Tox+ H. pylori exhibited marked vacuolation (52% +/- 5% of cells) compared with epithelial cells exposed to either CS from Tox- H. pylori (23% +/- 3.2%) or uninoculated control broth (23% +/- 3.7%) (P < 0.05) by light microscopy, which was confirmed by electron microscopy and antibody inhibition studies. These are the first data to show that H. pylori cytotoxin causes vacuolation of primary human mucosal epithelial cells.

MeSH Terms (12)

Bacterial Proteins Bacterial Toxins Cell Separation Cell Survival Cytotoxins Epithelium HeLa Cells Helicobacter pylori Humans Intestinal Mucosa Microscopy, Electron Vacuoles

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