Disruption of epithelial gamma delta T cell repertoires by mutation of the Syk tyrosine kinase.

Mallick-Wood CA, Pao W, Cheng AM, Lewis JM, Kulkarni S, Bolen JB, Rowley B, Tigelaar RE, Pawson T, Hayday AC
Proc Natl Acad Sci U S A. 1996 93 (18): 9704-9

PMID: 8790395 · PMCID: PMC38493 · DOI:10.1073/pnas.93.18.9704

Chimeric mice in which lymphocytes are deficient in the Syk tyrosine kinase have been created. Compared with Syk-positive controls, mice with Syk -/- lymphocytes display substantial depletion of intraepithelial gamma delta T cells in the skin and gut, with developmental arrest occurring after antigen receptor gene rearrangement. In this dependence on Syk, subsets of intraepithelial gamma delta T cells are similar to B cells, but distinct from splenic gamma delta T cells that develop and expand in Syk-deficient mice. The characteristic associations of certain T-cell receptor V gamma/V delta gene rearrangements with specific epithelia are also disrupted by Syk deficiency.

MeSH Terms (15)

Animals Electrophoresis, Polyacrylamide Gel Enzyme Precursors Epithelial Cells Epithelium Flow Cytometry Intracellular Signaling Peptides and Proteins Lymphocytes Mice Mutagenesis, Site-Directed Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Protein-Tyrosine Kinases Receptors, Antigen, T-Cell, gamma-delta Syk Kinase

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