Density of Helicobacter pylori infection in vivo as assessed by quantitative culture and histology.

Atherton JC, Tham KT, Peek RM, Cover TL, Blaser MJ
J Infect Dis. 1996 174 (3): 552-6

PMID: 8769613 · DOI:10.1093/infdis/174.3.552

Helicobacter pylori density was assessed by quantitative culture and histologic examination of gastric biopsy specimens from 29 H. pylori-infected dyspeptic patients. Density was correlated with cagA and vacA genotypes (assessed by polymerase chain reaction and colony hybridization), gastric inflammation and epithelial injury (assessed histologically), and peptic ulceration. Quantitative culture was more reproducible than histology, and antral density was more reproducible than corpus density. Mean antral density of cagA+/vacA sl strains was 4-fold higher than that of cagA-/vacA s2 strains (1.9 X 10(6) vs. 4.5 x 10(5) cfu/g, P = .02). Antral density was associated with mucosal neutrophilic and lymphocytic infiltration (P < .01) and with epithelial injury (P < .05). Mean antral bacteria] density was 5-fold higher in duodenal ulcer patients than in others (P = .005). In conclusion, H. pylori density in vivo is easily quantified and is associated with bacterial virulence determinants, gastric inflammation, and duodenal ulceration, suggesting a central role in pathogenesis.

MeSH Terms (13)

Antigens, Bacterial Bacterial Proteins Biopsy Gastritis Helicobacter Infections Helicobacter pylori Humans Nucleic Acid Hybridization Peptic Ulcer Polymerase Chain Reaction Prospective Studies Stomach Virulence

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