Immunophenotypic analysis of hematologic malignancy by laser scanning cytometry.

Clatch RJ, Walloch JL, Zutter MM, Kamentsky LA
Am J Clin Pathol. 1996 105 (6): 744-55

PMID: 8659450 · DOI:10.1093/ajcp/105.6.744

The authors tested a newly-developed computerized laser scanning cytometer (LSC) as a means of performing immunophenotypic analysis of hematologic specimens within their community hospital. Results were compared on a case-by-case basis with parallel flow cytometric and immunohistochemical data. A total of 71 specimens analyzed include 22 excised lymph nodes or other tissue biopsies, 18 peripheral bloods, 17 bone marrow aspirates, 7 body fluids, and 7 fine-needle aspiration biopsies of lymphoid tissue. The LSC proved to be a useful instrument capable of generating simultaneous two-color immunofluorescent data directly analogous to that obtained via conventional flow cytometry. However, laser scanning cytometric analysis provides advantages over flow cytometric analysis, because the LSC measures cells on a slide rather than in a fluid stream. Specifically, cells can be microscopically examined at any time--before, during, or after automated immunofluorescent analysis. In addition, specimen preparation techniques are less restricted and more cost efficient. Lastly, even extremely small and/or hypocellular specimens (such as body fluids and fine-needle aspiration biopsies) can be successfully analyzed.

MeSH Terms (8)

Diagnosis, Computer-Assisted Humans Image Cytometry Immunohistochemistry Immunophenotyping Lasers Leukemia Lymphoma

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