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Characterization of an N6-oxopropenyl-2'-deoxyadenosine adduct in malondialdehyde-modified DNA using liquid chromatography/electrospray ionization tandem mass spectrometry.

Chaudhary AK, Reddy GR, Blair IA, Marnett LJ
Carcinogenesis. 1996 17 (5): 1167-70

PMID: 8640930 · DOI:10.1093/carcin/17.5.1167

Malondialdehyde (MDA), a product of lipid peroxidation, causes mutations in bacterial and mammalian cells and cancer in rats. MDA reacts with deoxynucleosides in vitro and the monomeric adduct of MDA with deoxyguanosine (M1G-dR) is the major adduct. M1G-dR has been detected in rat and human liver. Random mutagenesis studies with MDA-modified DNA and recent 32P-postlabeling studies indicate that in addition to M1G-dR, adducts to deoxyadenosine may also be formed. We have utilized liquid chromatography coupled with electrospray ionization tandem mass spectrometry to characterize an N6-oxopropenyl-2'-deoxyadenosine adduct (M1A-dR) in calf DNA modified with MDA.

MeSH Terms (8)

Animals Chromatography, High Pressure Liquid Deoxyadenosines DNA Adducts Humans Malondialdehyde Mass Spectrometry Rats

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